Investigation protocals for
Primary
Secondary
Tertiary
Thanks in advance
Primary: nontreponemal serologic test (RPR or VDRL), Darkfield microspcopy of the lesion to see the spirochete. Direct antigen test ( MHA-TP or F TA-ABS) to confirm.
Secondary:Clinical symptoms plus positive serologic tests
Tertiary: I am not too sure
Tertiary: DIFFERENT system involvement such as CNS associated with positive serology