05-25-2018, 02:52 AM
Ok, so I need some help determining what unknown bacteria I have. I cannot repeat any tests. I have two in mind that I'm between but I'm not sure if I should mention them here yet....I would like a fresh perspective with nothing but the results to influence your thinking...
Here's what I have:
Gram neg rods
Not aerobic
Positive for glucose fermentation: supported by PRG and TSIA slant results
Positive for lactose fermentation: supported by EMB, Litmus Milk, PRL, and TSIA slant results
SIM tube: positive for H2S (also positive with PIA and the TSIA slants), positive for indole (also positive with Tryptone broth), positive for motility
positive for urease
negative for gelatinase
negative for deaminase
negative for lipase
negative for starch hydrolysis/amylase
negative for oxidase
positive for catalase
Nitrates reduced to Nitrites
MR positive/VP negative
Citrate negative
All tests were incubated at 37 C for 1 week...I know many tests are supposed to be incubated at 30 C for 48 hrs. This is how this particular class/school always does it. I'm not sure how much this would skew results. Any help is much appreciated!
Here's what I have:
Gram neg rods
Not aerobic
Positive for glucose fermentation: supported by PRG and TSIA slant results
Positive for lactose fermentation: supported by EMB, Litmus Milk, PRL, and TSIA slant results
SIM tube: positive for H2S (also positive with PIA and the TSIA slants), positive for indole (also positive with Tryptone broth), positive for motility
positive for urease
negative for gelatinase
negative for deaminase
negative for lipase
negative for starch hydrolysis/amylase
negative for oxidase
positive for catalase
Nitrates reduced to Nitrites
MR positive/VP negative
Citrate negative
All tests were incubated at 37 C for 1 week...I know many tests are supposed to be incubated at 30 C for 48 hrs. This is how this particular class/school always does it. I'm not sure how much this would skew results. Any help is much appreciated!