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+--- Thread: cell and mol bio q4 - basicq (/showthread.php?tid=515082)
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cell and mol bio q4 - basicq - ArchivalUser - 06-24-2010
A woman trying to get pregnant has a sister whose child has an autosomal recessive disease characterized by dysfunction of mucus-secreting cells. As a result, this child has abnormally thick mucus that tends to precipitate into dense plugs that obstruct the pancreatic ducts, bronchi, and bronchioles, as well as the bile ducts. Which of the following tests could be performed to determine if this woman and her husband are carriers of this disease?
A.
Northern blot
B.
PCR and sequencing
C.
Southern blot
D.
Western blot
0 - ArchivalUser - 06-24-2010
cyctic fibrosis do DNA squencing by Southern blot CCCCCCCCCCCC
0 - ArchivalUser - 06-24-2010
its northan blot
hey haval why r u confusing?
in previous u have mentioned northen blot for cystic fibrosis and here u mention southern blot
hw could?
0 - ArchivalUser - 06-24-2010
B..
0 - ArchivalUser - 06-24-2010
Dna ..so southern blot??
0 - ArchivalUser - 06-24-2010
If you want to see amplification region where the mutation occurs, either in cystic fibrosis or any other disease... you have to do polymerase chain reaction and run that PCR product in a DNA gel electrophoresis to see the DNA size. The band in the DNA gel can then be sequenced in a sequencer and give the exact idea.
Answer is B
0 - ArchivalUser - 06-24-2010
agree with drrock. PCR then sequencing.
0 - ArchivalUser - 06-24-2010
PCR The amplification of this region using PCR can be done and then that portion to compare to normal sequence.
0 - ArchivalUser - 06-24-2010
B.
0 - ArchivalUser - 06-25-2010
yes bbb