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Biochem Q - forward
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03-12-2007, 08:36 AM
The peptide Leu-Glu-Lys-Ser-Pro-Cys would be cut into two peptides most efficiently by
a. trypsin b. chymotrypsin c. elastase d. none of the above
03-12-2007, 01:19 PM
Trypsin - cuts after Arg or Lys, unless followed by Pro.
Chymotrypsin - cuts after Phe, Trp, or Tyr, unless followed by Pro. Cuts more slowly after Asn, His, Met or Leu. Elastase - cuts after Ala, Gly, Ser, or Val, unless followed by Pro. Pepsin - cuts *before* Leu, Phe, Trp or Tyr, unless *preceded* by Pro. Therefore it is A.
03-13-2007, 06:00 AM
how come your guys can remember these?
If I have a question like this, I would faint immediately.
03-13-2007, 06:18 AM
well if those details are required for answering questions.....it involves knowing far more detail...than i store in my memory bank for one exam.......
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