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A geneticist has artificially produced a microorganism that contains a new combination of genes. He wants to know if recombination or transposition has occurred. The major difference between these two processes is that recombination involves which of the following?
A) DNA repair
B) Endonuclease sites
C) Gene rearrangement
D) Homologous sites
E) Sites on the same chromosome
im getting confused bet BBBBB or DDDDDDDDDD
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terminology--i think
1.homologous recombination
2.transposition recombination or site specific recombination.
here in the q they gave recombination vs transposition, and asking for recombination so i would assume this as homologous recombination,then in that case homologous recombination needs homology reqiured, D IS THE ANSWER.
if it would have been asked for transposition then restrictive endonuclease is needed to get in the cromosome then B is right in that case.
but both are recombination process tricky???
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thanq sodium 4 ur reply...
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if u know d coreect ans...send me the post, thanq in advance
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KAPLAN MICRO 2008 PAGE 469 AND 490
As sodium explained
Homologous Recombination
-is a mechanism to incorporate short linear pieces of DNA into the chromosome
-There must be SOME SEQUENCE HOMOLGY(CHOICE D)
-Recombinase A is required
-There is ONE TO ONE EXCHANGE of DNA
Transposition(the name of SITE SPECIFIC INTEGRATION OF TRANSPOSONS)
-is the mechanism used to combine circular pieces of TRANSPOSONS
-It requires NO HOMOLGY(CHOICE D)
-No DNA is lost
-IT REQUIRES RESTRICTION RESTRICTION ENDONUCLEASES
hence my answer is DDDD ..as sodium explained it
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thanq......sodium & yeabiruh
