03-08-2011, 08:56 PM
Which of the following is NOT required for a PCR reaction?
a) A thermostable DNA polymerase
b) Dideoxy-dNTPs (ddNTPs)
c) Template DNA
d) Primers
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bioch. Q - raheem
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 Which of the following is NOT required for a PCR reaction? a) A thermostable DNA polymerase b) Dideoxy-dNTPs (ddNTPs) c) Template DNA d) Primers
03-08-2011, 09:05 PM
Which of the following is NOT required for a PCR reaction? Your Answer: b) Dideoxy-dNTPs (ddNTPs) Feedback: Dideoxy-dNTPs, which lack a 3' OH on the ribose, cause chain termination if they are present in a DNA synthesis reaction so they are not used in PCR. They are used in DNA sequencing. A PCR reaction requires a template, dNTPs, a thermostable (heat-tolerant) DNA polymerase and primers. Page reference: 462-463 |
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