q_____________? - sarim

[ArchivalUser]

A couple is expecting their first child have consulted a genetic counselor because the woman has a brother who died of sickle cell anemia.There is also hx of disease in the man's family.Fetal cells are obtained by amniocentesis.which test would be best determine whether the fetus would be born with the disease?

A-Hb electrophoresis
B-Western Blot
C-Northern blot
D-PCR with allele-specific probes on dot blot

[ArchivalUser]

This looks so familiar!!!! I cant recall but i kno

SS = valine in replace of glutamtamic acid....
and its a defective shape...

so AAA ?? this is my work up hopefully im right this time lol.. thank you sarim!

[ArchivalUser]

AA..

agree with zen!

[ArchivalUser]

nope.

[ArchivalUser]

u cant detect HbSS until 6months of age so

?D

[ArchivalUser]

D?

[ArchivalUser]

OH man Sarim your questions are really good / tough!!

BB ? protein based amino acid ? ...

[ArchivalUser]

DD

[ArchivalUser]

NOTE:

beta-Globulin gene is "NOT Expressed" during Fetal life.It's expression occur after the birth.

so at this stage the Fetus has Neither "beta-globulin (protein)" Nor "beta-globulin RNA transcript"

the only thing fetus got is the "beta-globulin Gene"(DNA) itself.

so in order for us to assess the defect, we'll have to test the DNA-strand.(* option D)

-Option A, B, and C are when a gene is Expressed.

thanks