06-19-2012, 05:20 AM
During an investigational study, an assay is set up to determine the activity of hypothetical enzyme in biochemical samples. Enzyme activity is measured by adding increasing amounts of substrate to an otherwise optimal incubation system. The data obtained are shown:
[S] (mM) Vo (mmol/sec)
0.10 0.20
0.30 0.60
0.50 1.00
0.70 1.22
1.00 1.51
1.20 1.66
1.50 1.90
2.00 2.15
2.50 2.29
3.00 2.33
Which of the following substrate concentrations is most suitable for conducting the desired enzyme assays?
A- 0.30 mM
B- 1.20 mM
C-1.50 mM
D- 2.00 mM
E- 3.00 mM
Please, can someone give me the explanation? I would choose A but the answer is E. I don,t understand. Please explanation, thank u
[S] (mM) Vo (mmol/sec)
0.10 0.20
0.30 0.60
0.50 1.00
0.70 1.22
1.00 1.51
1.20 1.66
1.50 1.90
2.00 2.15
2.50 2.29
3.00 2.33
Which of the following substrate concentrations is most suitable for conducting the desired enzyme assays?
A- 0.30 mM
B- 1.20 mM
C-1.50 mM
D- 2.00 mM
E- 3.00 mM
Please, can someone give me the explanation? I would choose A but the answer is E. I don,t understand. Please explanation, thank u
cheers