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A scientist wishes to insert her gene of interest into a yeast vector. She decides to use a restriction endonuclease that cleaves the vector at a palindromic recognition site leaving sticky ends. Which of the following is a possible recognition site for the restriction enzyme?
A. 5'-AAGCTA, 3'-TTCGAT
B. 5'-GCATTC, 3'-CGTAAG
C. 5'-CAATTG, 3'-GTTAAC
D. 5'-GGATCG, 3'-CCTAGC
E. 5'-TCAGGA, 3'-AGTCCT
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i dont get how r these sequences written
can anyone explain
y is 3' in the middle of the sequence???
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oops sorry!
yes CCCCCCCCCC
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C is the correct answer
Don't worry -- you don't need to memorize the recognition sequences! You simply need to know that restriction enzymes that splice at recognition sites are almost always palindromic. There are some restriction enzymes that cut outside the recognition site; these do not necessarily have palindromic recognition sites. Here, 5'-CAATTG & 3'-GTTAAC is the only answer choice with a pallindromic sequence
