nbme 4 block 2 question 1 - usmle123go

[ArchivalUser]

1. Which of the following best explains the purpose of including antibiotic-resistant genes in plasmids used as vectors for cloning human genes?
A) Facilitates growth of the bacterial plasmid in human cells
B) Permits selection against bacterial cells that do not acquire a plasmid
C) Prevents the clones from growing outside of the laboratory
D) Regulates expression of the inserted human gene

[ArchivalUser]

Is that the one missing?

[ArchivalUser]

yes

[ArchivalUser]

hi

answer is BBB

The reason why the vector must have at least one gene resistance to an antibiotic cz when we place that vector on the growth medium, we also add antibiotic to that medium so it can kill all those bacterias which did not acquire the cloning vector and since our cloning vector got resistace gene against the added antibiotic , those bacterias which acquired it will be spared and produce a culture.