question - eu_phoria

[ArchivalUser]

cloning of a new eukaryotic gene was followed by insertion and ligation of the gene in an expression vector . The protein translated from this was then studied on a western blot and probed with 32 P-DNA, yielding a positive result. These findings eliminate which of the following substances as a candidate for the likely gene product?

1] CREB
2] Protein Kinase A
3] RNA Polymerase
4] SP-1
5] Steroid Receptor

please explain the answer.

[ArchivalUser]

B. Protein Kinase A. This is gel-shift assay to Identify Protein-DNA Interactions in the nucleus. PKA is in cytoplasm and does not interact with DNA.