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NBME form 3 block 1 question 12...please help!!! - rim0209
#3
the answer id D..Transformation....

Answer by yeabiruh.

--Bacterial X =Ampicillin Resisitant and Gentamicin Sensitive
--Bacterial y=Ampicillin Sensitive and Gentamicin Resisitant

--First Experimen =No DNase added and X and Y grow in mixed CULTURE with Amp and Gen
outcome=colnies GROW

--Second Experiment=DNase added and X and Y grow in mixed culture with Amp and Gen
outcome =no colnies GROW

--the difference between the first and second experiment is the ADDITION of DNase

--DNase breaks down naked Chromosomes ..

--In the first experiment there is growth because the bacterias can take naked DNA from the suurounding By TRANSFORMATION =gets the antibiotic resistance

--in the second experiment there is no GROWTH because there is no AVAILABLE DNA to be taken from the environment by TRANSFORMATION due to the action of DNase=NO antibiotic resistance =no growth

hence my answer dddd
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