Vit. Q - pth

[ArchivalUser]

actually this enzyme also requires-NAD,FAD,CoA,LIPOIC ACID in addition to TPP..-SIMILAR TO PDH COMPLEX AND ALPHA KGDH...

[ArchivalUser]

Vomit----Valine, Odd carbon FA, Methionine, Isoleucine, Threonine...all these AAcids are precursor for succinyl CoA and require methyl malonyl CoA mutase enzyme (Cofactor B12) for their last step of metabolism.........right??

[ArchivalUser]

Direct physical evidence for stabilization of branched-chain alpha-ketoacid dehydrogenase by thiamin pyrophosphate.

Heffelfinger SC, Sewell ET, Elsas LJ, Danner DJ.

Branched-chain alpha-ketoacid dehydrogenase is a multienzyme complex composed of four subunits. The 46,500-dalton protein is a subunit of the decarboxylase component, which is selectively digested by chymotrypsin. Two peptides of apparent mol. wts. of 36,000 and 15,000 result with loss of enzyme activity. When the complex is saturated with thiamin pyrophosphate and ketoacid substrate, digestion by chymotrypsin does not occur. These data provide direct physical evidence for the stabilization of the complex by the presence of the vitamin B1-derived cofactor.

[ArchivalUser]

I see now for the first step needs thimine, L acid, Coa, FAD, NAD but for last stage of metabolism needs B12.....thanks guys