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cell and mol biology q3 - basicq
#1
a gene product involved in down regulation of fetal Hb expression is being investigated.Samples of primary tissue cultures of fetal,neonatal, and adult liver and bone marrow as well as adequet amount of DNA probe believed to contain the studied gene,are provided.The best method for determining which of the tissue culture samples expresses the studied gene is

a)DNA SEQUENCING
b)NORTHERN BLOT
c)PCR
d)SOUTHERN BLOT
e)WESTERN BLOT

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#2
BBBB expressing the gene means produing the mRNA.
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#3
DD
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#4
BBBB....
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#5
bbbbbbbb
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#6
BB
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#7
BB

D - southern blot - that would tell you if they have the gene in their DNA sequence but not necessarily expression.
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#8
B. the only method that can tell a gene expressed or not.
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#9
yep bb
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