Histo Q....1 - psychmledr

[ArchivalUser]

A researcher is examining the distribution of an ion channel protein in the kidney. She incubates slices of kidney tissue in a dilute solution of a specific antibody directed again the protein, then uses the immunoperoxidase method to localize the ion channel proteins. She notes the presence of brown pigment in a population of epithelial cells, which on closer examination, have a brush border. The researcher concludes that the protein is probably present in cells of which of the following?
a. Collecting duct
b. Deep portion of loop of Henle
c. Distal convoluted tubule
d. Glomerulus
e. Proximal convoluted tubule

[ArchivalUser]

e- PCT=bush border

[ArchivalUser]

eee to reabsorbs glucose

[ArchivalUser]

Answer is E

The immunoperoxidase method uses horseradish peroxidase to produce a visible pigment when a specific antibody binds to antigenic sites in the tissue. Binding of the antibody to the epithelial cells indicates that the protein in question is being expressed by those cells. The positive immunoperoxidase results obtained here suggest that the channel protein is expressed in proximal convoluted tubular epithelium, since this is the only site in the kidney at which the epithelial cells have a brush border. The brush border is made of microvilli, which enhance the proximal tubules™ ability to reabsorb plasma constituents filtered at the gomeruli.

[ArchivalUser]

nice qs psych... whats the source of these?
and how have u all been doing? been a while!

[ArchivalUser]

er 12!! Where have you been? I've posted a thread ...looking for you... I thought you already took the test...or went to get married......or something