To goober(nbme form 3,part 1) - shwe

[ArchivalUser]

. Which of the following techniques is most accurate in showing that two bacterial DNAs are identical?

A. Hybridization
B. Northern blotting
C. Nucleotide sequencing
D. Polymerase chain reaction
E. Restriction enzyme analysis

[ArchivalUser]

C. Only nucleotide sequencing can determine the exact base sequence of each bacterial DNA.

Two similar but not identical DNA strands can hybridize together.

Northern blotting is used for RNA, not DNA.

PCR is a way of amplifying DNA if you know enough of the sequence to make a short primer, but if the sequences differ after the primer PCR will not tell you that.

Finally two DNA strands can have similar restriction patterns but differ in the sequences that the restriction enzymes don't cut.